4/11/2023 0 Comments Dcm 130 drivers![]() coli knockout mutants, one with deficiency in glycolysis – pgi (LC1888) and another inhibiting the oxidative pentose phosphate pathway (OPPP) – zwf (LC1889), were grown on glucose and used as a comparison against the wild type E. We measured δ 2H and δ 13C in amino acids in the wild type Escherichia coli (MG1655) across a range of growth rates in chemostat cultures to address the unknown isotopic consequences as metabolic fluxes are shuffled between catabolic and anabolic metabolisms. Here, we explore the effects that central carbon metabolism and growth rate can have on stable hydrogen (δ 2H) and carbon (δ 13C) compound specific isotopic values of amino acids, and whether diagnostic isotopic fingerprints are revealed by these paired analyses. By comparing isotopic values and fractionations relative to water and growth substrate, the impact of variable flow path for metabolites through different central metabolic pathways, perturbations of these paths, and their resultant consequences on intracellular pools and resultant biomass may be elucidated. Compound specific stable isotope analysis (CSIA) of amino acids from bacterial biomass is a newly emerging powerful tool for exploring central carbon metabolism pathways and fluxes.
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